Monday, March 8, 2010

Chapter 20: Biotechnology

Q: What is a stem cell?

A: It is a relatively unspecialized cell that both reproduce itself indefinitely and, under appropriate conditions, differentiate into specialized cells of one or more types.

Q: What is a genome library?

A: The complete set of plasmid-containing cell clones, each carrying copies of a particular segment from the initial genome.

Q: Then, what are plasmids?

A: Small circular DNA molecules that replicate separately from the bacterial chromosome.

1. DNA cloning yields multiple copies of a gene or other DNA segment

2. DNA technology allows us to study the sequence, expression, and function of a gene.

3. Cloning organisms may lead to production of stem cells for research and other applications.

4. The practical applications of DNA technology affect our lives in many ways.

5. Many early animal embryos contain stem cells capable of giving rise to differentiated embryonic cells of any type.



Single nucleotide polymorphisms as genetic markers for disease-causing alleles. This diagram depicts homologous segments of DNA from a family in which some members have a genetic disease. In this family, unaffected family members have a T at a particular SNP locus. If a family member has a C at that locus, there is a high probability that the individual has also inherited the disease-causing allele.

It is the genetic manipulation of organisms and humans have been doing this for thousands of years. Basic tools of bioengineering include restriction enzymes, ligase, plasmids, DNA libraries and advanced tools include PCR, DNA sequencing, gel electrophoresis, southern blotting, etc.
Word processing metaphors are used, and they are cut, paste, copy, and find.
There have been many experiments cloning organisms, like plants and animals. Also, some of the applications can affect our lives, like forensic evidence and genetic profiles, environment, agriculture, or in medical world.

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